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    José María Eiros MD PhD, María Rosario Bachiller MD,
    Raul Ortiz de Lejarazu MD PhD, Antonio Rodríguez MD PhD.

    Areas de Microbiología y Pediatría. Facultad de Medicina. Hospital Universitario. Valladolid. Spain

    Rev Electron Biomed / Electron J Biomed 2004;3:52-53.

    To the Editor

    The importance acquired by the strategy of anticipating rubella revacunation needs no emphasis1. Recommendations by experts committees and actualization of the prevailing childhood immunization schedules are unanimous when it comes to including the above-mentioned strategy2. In this context, seroepidemiologic survey studies allow the assesment of humoral immunologic response against viral structural antigens3,4 despite the assumed potential bias attached to their design. Being aware of the importance of describing rubella seropositivity real status in our setting we decided to document such situation in children under theoretically proper immunization schedules. The present contribution relies on data from a cross-sectional study carried out between 1999 and 2000 in children from the autonomous region of Spain, Castilla y León, (the largest region in the European Economic Community). The chosen framework was restricted to serum samples received in a Microbiology Laboratory of a University Hospital with the request of analyzing infectious markers other than rubella antibodies. According to demographic features a double stratification was made and we evaluated 323 children whose age was between 1 and 5 years and 1166 from 6 to 14 years of age. All samples were alicuoted and frozen (-20ºC) until the moment of processing. Antibodies to proteic antigens of rubella viral envelope were determined by means of an indirect enzymeimmunoassay (EIA) (Bio-Whittaker, USA). Results were validated according to manufacturer's instructions and samples that showed a neat absorbance greater the cut-off value plus 15% were considered positive.

    Our findings reveal that 309 of the investigated samples belonging to children from 1 to 5 years of age (95.7%) had antibodies to rubella virus (95% CI, 93.2% to 98.2%), being the rest of them seronegative at the moment of our study. In the age group ranging from 6 to 14 years the prevalence of antibodies was 90.5% (1055/1166) (95% CI, 88.6% to 92.5%) which resulted lower to that found in the previous group and the difference was statistically significant (p=0.003). This fact reflects a decrease of 5.2% in the seropositivity percentage of the second age group. An additional finding was that when we investigated the 6 to 14 years old group in terms of gender differences, girls (568) reached significantly higher percentages of seroprotection than boys (521) did (94.4 vs 87.1%, p=0.000).

    Conscious of the caution required in this kind of studies and assuming the internal validity for the evaluated population, our results point out to a age dependent loss of seroprotection. Among the potential causes that maintain this fact we may denote on the one hand the differences in vaccine coverage reached by both groups of children, just as Davidkina et al.5 have recently reported, and on the other hand limitations inherent in the vaccination itself6,7. Moreover, it is of course true that in our country, as well as in all developed countries, systematic vaccination of girls before puberty yields high efficiency, in order to prevent consequences derived from a potential primary infection during pregnancy8,9, all the same it is also certain that we still attend to the presence of small proportions of unprotected persons. The efficiency of new strategies of anticipating combined vaccines needs to be evaluated, and seroepidemiological studies seem to be a good tool for such purpose10, being the detection of antibodies by means of EIA the most sensible method for this aim.


      1. De Haas R, Van Der Horf S, Berbers GA, De Melker HE, Conyn-Van Spaendock MA. Prevalence of antibodies against rubella virus in The Netherlans 9 year after changing from selective to mass vaccination. Epidemiol Infect 1999; 123: 263-70.

      2. Irons B, Lewis MJ, Dahl-Regis M, Castillo-Solorzano C, Carrasco PA, De Quadros CA. Strategies to eradicate rubella in the English-speakung Caribbean. Am J Public Health 2000; 90: 1545-9.

      3. Chakravarti A, Yadav S, Berry N, Rastogi A, Mathur MD. Evaluation of serological status of rubella & mumps in children below five years. Indian J Med Res 1999; 110: 1-3.

      4. Hofmann J, Gerstenberger S, Lachmann I, Atreya CD, Liebert UG. Rubella virus nonstructural protein 2 is a minor immunogen. Virus Res 2000; 68: 155-60.

      5. Davidkina I, Peltolab H, Leinikkia P, Vallea M. Duration of rubella immunity induced by two-dose measles, mumps and rubella (MMR vaccination. A 15 year follow-up in Finland. Vaccine 2000; 18: 3106-12.

      6. Crovari P, Gabutti G, Giammanco G, Dentico P, Moiraghi AR, Ponzio F et al. Reactogenicity and immunogenicity of a new combined measles-mumps-rubella vaccine: results of a multicentre trial. The Cooperative Group for the Study of MMR vaccines. Vaccine 2000; 18: 2796-803.

      7. Klinge J, Lugauer S, Korn K, Heininger U, Stehr K. Comparison of immunogenicity and reactogenicity of a measles, mumps and rubella (MMR) vaccine in German children vaccinated at 9-11, 12-14 or 15-17months of age. Vaccine 2000; 18: 3134-40.

      8. Anonymous. Preventing congenital rubella syndrome. Wkly Epidemiol Rec 2000; 75: 290-5.

      9. Lawn JE, Reef S, Baffoe-Bonnie B, Adadevoh S, Caul EO, Griffin GE. Unseen blindness, unheard deafness, and unrecorded death and disability: congenital rubella in Kumasi, Ghana. Am J Public Health 2000; 90: 1555-61.

      10. Cutts FT, Abebe A, Messele T, Dejene A, Enquselassie F, Nigatu WT al. Sero-epidemiology of rubella in the urban population of Addis Ababa, Ethiopia. Epidemiol Infect 2000; 124: 467-79.

    Correspondence: José Mª Eiros.
    Area de Microbiología. Facultad de Medicina.
    Avda Ramón y Cajal, 7. 47005 Valladolid. Spain.

    Received December 12, 2004.
    Published December 15, 2004